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electric cell substrate impedance sensing ecis zθ instrument  (Applied BioPhysics)


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    Applied BioPhysics electric cell substrate impedance sensing ecis zθ instrument
    Electric Cell Substrate Impedance Sensing Ecis Zθ Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 637 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electrical+cell-substrate+impedance+sensing+(ecis)+instrument/ECIS+Z+Theta/10__1096_slash_fj__202402767r-61-10-18
    Average 96 stars, based on 637 article reviews
    electric cell substrate impedance sensing ecis zθ instrument - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Attachment Assay:

    Article Title: Fibulin‐4 and latent‐transforming growth factor beta‐binding protein‐4 interactions with syndecan‐2 and syndecan‐3 are required for elastogenesis
    Article Snippet: The dye was solubilized for 10 min in 10% acetic acid in TBS on a shaker at 500 rpm, and the absorbance was determined at 570 nm (Beckman Coulter, Model# DTX 880). .. To analyze cell attachment in real- time, we used an Electric Cell- Substrate Impedance Sensing (ECIS) Zθ instrument (Applied Biophysics) operated at 37°C at 5% CO2. .. The cells were analyzed in ECIS 96- well plates (Applied Biophysics; #96W20idf PET).

    Article Title: Fibulin‐4 and latent‐transforming growth factor beta‐binding protein‐4 interactions with syndecan‐2 and syndecan‐3 are required for elastogenesis
    Article Snippet: The dye was solubilized for 10 min in 10% acetic acid in TBS on a shaker at 500 rpm, and the absorbance was determined at 570 nm (Beckman Coulter, Model# DTX 880). .. To analyze cell attachment in real‐time, we used an Electric Cell‐Substrate Impedance Sensing (ECIS) Zθ instrument (Applied Biophysics) operated at 37°C at 5% CO 2 . .. The cells were analyzed in ECIS 96‐well plates (Applied Biophysics; #96W20idf PET).

    Electric Cell-substrate Impedance Sensing:

    Article Title: Fibulin‐4 and latent‐transforming growth factor beta‐binding protein‐4 interactions with syndecan‐2 and syndecan‐3 are required for elastogenesis
    Article Snippet: The dye was solubilized for 10 min in 10% acetic acid in TBS on a shaker at 500 rpm, and the absorbance was determined at 570 nm (Beckman Coulter, Model# DTX 880). .. To analyze cell attachment in real- time, we used an Electric Cell- Substrate Impedance Sensing (ECIS) Zθ instrument (Applied Biophysics) operated at 37°C at 5% CO2. .. The cells were analyzed in ECIS 96- well plates (Applied Biophysics; #96W20idf PET).

    Article Title: Fibulin‐4 and latent‐transforming growth factor beta‐binding protein‐4 interactions with syndecan‐2 and syndecan‐3 are required for elastogenesis
    Article Snippet: The dye was solubilized for 10 min in 10% acetic acid in TBS on a shaker at 500 rpm, and the absorbance was determined at 570 nm (Beckman Coulter, Model# DTX 880). .. To analyze cell attachment in real‐time, we used an Electric Cell‐Substrate Impedance Sensing (ECIS) Zθ instrument (Applied Biophysics) operated at 37°C at 5% CO 2 . .. The cells were analyzed in ECIS 96‐well plates (Applied Biophysics; #96W20idf PET).

    Article Title: Comparative Analysis of microRNAs that Stratify in vitro Mammary stem and Progenitor Activity Reveals Functionality of Human miR-92b-3p.
    Article Snippet: and is supported by tissue-resident mammary stem and progenitor cells [1].. Considering their central role in fundamental processes of mammary gland biology and pathophysiology, mammary stem/progenitor cells are a focus of intense study.. The existence of mammary stem/progenitor cells in all mammals can be inferred from the ability of their glands to regenerate; however, these cells have only been identified and studied in a few species, including rodents, humans, and to a lesser extent cows and horses [reviewed in [2]].

    Article Title: Protective effects of lactic acid bacteria on gut epithelial barrier dysfunction are Toll like receptor 2 and protein kinase C dependent
    Article Snippet: .. Subsequently, T84 cells were seeded and cultured in precoated plates as described earlier.9 Before starting stimulation experiments, plates were first installed in the Electric Cell–substrate Impedance Sensing (ECIS) Ztheta instrument (Applied Biophysics) to monitor TEER at multiple frequencies for at least 5 h.10 Experiments were only performed with cells reaching stable TEER (around 1000 Ohm at 4000 Hz). .. T84 cells were first stimulated with various LAB strains (4 × 106 CFU per well) for 24 h. Afterwards, barrier disruptor calcium ionophore A23187 (Sigma-Aldrich, St Louis, USA) or deoxynivalenol (DON; Sigma-Aldrich, St Louis, USA) was applied in T84 cells to induce epithelial barrier defects.

    Article Title: Protective effects of lactic acid bacteria on gut epithelial barrier dysfunction are Toll like receptor 2 and protein kinase C dependent.
    Article Snippet: .. Subsequently, T84 cells were seeded and cultured in precoated plates as described earlier.9 Before starting stimulation experiments, plates were first installed in the Electric Cell–substrate Impedance Sensing (ECIS) Ztheta instrument (Applied Biophysics) to monitor TEER at multiple frequencies for at least 5 h.10 Experiments were only performed with cells reaching stable TEER (around 1000 Ohm at 4000 Hz). .. T84 cells were first stimulated with various LAB strains (4 × 106 CFU per well) for 24 h. Afterwards, barrier disruptor calcium ionophore A23187 (Sigma-Aldrich, St Louis, USA) or deoxynivalenol (DON; Sigma-Aldrich, St Louis, USA) was applied in T84 cells to induce epithelial barrier defects.

    other:

    Article Title: Dependence of ABCB1 transporter expression and function on distinct sphingolipids generated by ceramide synthases-2 and -6 in chemoresistant renal cancer.
    Article Snippet: Cells (1.5 × 105) grown in 8W10E arrays (Ibidi) were monitored using an ECIS1600R (ECIS, electric cell–substrate impedance sensing) instrument (Applied BioPhysics, Inc) (48), and ECIS software, version 1.2.186.0 PC.

    Article Title: Dependence of ABCB1 transporter expression and function on distinct sphingolipids generated by ceramide synthases-2 and -6 in chemoresistant renal cancer
    Article Snippet: Cells (1.5 × 10 5 ) grown in 8W10E arrays (Ibidi) were monitored using an ECIS1600R (ECIS, electric cell–substrate impedance sensing) instrument (Applied BioPhysics, Inc) , and ECIS software, version 1.2.186.0 PC.

    Article Title: Direct Targeting of the mTOR (Mammalian Target of Rapamycin) Kinase Improves Endothelial Permeability in Drug-Eluting Stents-Brief Report.
    Article Snippet: Transendothelial electrical resistance (TEER): Electrical resistance across the endothelial cell monolayer was measured using the Electric Cell-substrate Impedance Sensing (ECIS) instrument (Applied Biophysics, Troy, NY) and the ECIS V1.2.193.0 software (Applied Biophysics), according to the manufacturer’s instructions.

    Migration:

    Article Title: Comparative Analysis of microRNAs that Stratify in vitro Mammary stem and Progenitor Activity Reveals Functionality of Human miR-92b-3p.
    Article Snippet: and is supported by tissue-resident mammary stem and progenitor cells [1].. Considering their central role in fundamental processes of mammary gland biology and pathophysiology, mammary stem/progenitor cells are a focus of intense study.. The existence of mammary stem/progenitor cells in all mammals can be inferred from the ability of their glands to regenerate; however, these cells have only been identified and studied in a few species, including rodents, humans, and to a lesser extent cows and horses [reviewed in [2]].

    Cell Culture:

    Article Title: Protective effects of lactic acid bacteria on gut epithelial barrier dysfunction are Toll like receptor 2 and protein kinase C dependent
    Article Snippet: .. Subsequently, T84 cells were seeded and cultured in precoated plates as described earlier.9 Before starting stimulation experiments, plates were first installed in the Electric Cell–substrate Impedance Sensing (ECIS) Ztheta instrument (Applied Biophysics) to monitor TEER at multiple frequencies for at least 5 h.10 Experiments were only performed with cells reaching stable TEER (around 1000 Ohm at 4000 Hz). .. T84 cells were first stimulated with various LAB strains (4 × 106 CFU per well) for 24 h. Afterwards, barrier disruptor calcium ionophore A23187 (Sigma-Aldrich, St Louis, USA) or deoxynivalenol (DON; Sigma-Aldrich, St Louis, USA) was applied in T84 cells to induce epithelial barrier defects.

    Article Title: Protective effects of lactic acid bacteria on gut epithelial barrier dysfunction are Toll like receptor 2 and protein kinase C dependent.
    Article Snippet: .. Subsequently, T84 cells were seeded and cultured in precoated plates as described earlier.9 Before starting stimulation experiments, plates were first installed in the Electric Cell–substrate Impedance Sensing (ECIS) Ztheta instrument (Applied Biophysics) to monitor TEER at multiple frequencies for at least 5 h.10 Experiments were only performed with cells reaching stable TEER (around 1000 Ohm at 4000 Hz). .. T84 cells were first stimulated with various LAB strains (4 × 106 CFU per well) for 24 h. Afterwards, barrier disruptor calcium ionophore A23187 (Sigma-Aldrich, St Louis, USA) or deoxynivalenol (DON; Sigma-Aldrich, St Louis, USA) was applied in T84 cells to induce epithelial barrier defects.



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    The OGR1 inhibitor blocked OGR1-associated downstream events in Caco-2 cells and human CD14+ monocytes. ( A ) OGR1 (GPR68) antibody 16H23L16 for immunocytochemical detection of OGR1 was verified in previously validated tools. Caco-2 clone stably overexpressing OGR1 (clone U1); negative control, Caco-2 vector control (VC) cells. Cells were examined by confocal microscopy (Leica, Germany). ( B – F ) Functional assays confirmed pH-dependent OGR1-mediated signalling. ( B ) U1 and VC cells were subjected to an acidic pH shift and intracellular inositol phosphate (IP) formation was measured. Data are presented as mean ± SD and statistical analysis was performed using one-way ANOVA. *** p < 0.001. ( C ) Activated GTPase RhoA was measured in U1 and VC cells upon an acidic pH shift. ( D , E ) Efficacy of the OGR1 inhibitor (GPR68-I) and the enantiomer of the inhibitor were measured using electric cell-substrate impedance-sensing <t>(ECIS)</t> technology. OGR1 clone U1 and VC grown to confluent monolayers were subjected to an acidic pH shift with or without the OGR1 inhibitor (0.5, 1.0, 2.5, 5, 10, 25 µM). Changes in resistance of cell monolayers were monitored in real time. Representative graph of eight independent experiments shown for the inhibitor or the enantiomer. ( F , G ) Human CD14+ monocytes from healthy volunteers and active IBD patients were subjected to an acidic pH shift, with or without the OGR1 inhibitor (0.5, 1.0, 2.5, 5 µM). IP1 production was measured from healthy volunteers ( n = 5) and active IBD patients ( n = 2); active IBD patient ( n = 1). Samples in ( F , G ) were read on the Tecan Infinite or Biotek Synergy instrument, respectively. Data are presented as mean ± SD and statistical analysis was performed using one-way ANOVA. * p < 0.05. IBD: inflammatory bowel disease; OGR1: ovarian cancer G-protein-coupled receptor 1; vector control: VC.
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    Image Search Results


    The OGR1 inhibitor blocked OGR1-associated downstream events in Caco-2 cells and human CD14+ monocytes. ( A ) OGR1 (GPR68) antibody 16H23L16 for immunocytochemical detection of OGR1 was verified in previously validated tools. Caco-2 clone stably overexpressing OGR1 (clone U1); negative control, Caco-2 vector control (VC) cells. Cells were examined by confocal microscopy (Leica, Germany). ( B – F ) Functional assays confirmed pH-dependent OGR1-mediated signalling. ( B ) U1 and VC cells were subjected to an acidic pH shift and intracellular inositol phosphate (IP) formation was measured. Data are presented as mean ± SD and statistical analysis was performed using one-way ANOVA. *** p < 0.001. ( C ) Activated GTPase RhoA was measured in U1 and VC cells upon an acidic pH shift. ( D , E ) Efficacy of the OGR1 inhibitor (GPR68-I) and the enantiomer of the inhibitor were measured using electric cell-substrate impedance-sensing (ECIS) technology. OGR1 clone U1 and VC grown to confluent monolayers were subjected to an acidic pH shift with or without the OGR1 inhibitor (0.5, 1.0, 2.5, 5, 10, 25 µM). Changes in resistance of cell monolayers were monitored in real time. Representative graph of eight independent experiments shown for the inhibitor or the enantiomer. ( F , G ) Human CD14+ monocytes from healthy volunteers and active IBD patients were subjected to an acidic pH shift, with or without the OGR1 inhibitor (0.5, 1.0, 2.5, 5 µM). IP1 production was measured from healthy volunteers ( n = 5) and active IBD patients ( n = 2); active IBD patient ( n = 1). Samples in ( F , G ) were read on the Tecan Infinite or Biotek Synergy instrument, respectively. Data are presented as mean ± SD and statistical analysis was performed using one-way ANOVA. * p < 0.05. IBD: inflammatory bowel disease; OGR1: ovarian cancer G-protein-coupled receptor 1; vector control: VC.

    Journal: International Journal of Molecular Sciences

    Article Title: pH-Sensing G Protein-Coupled Receptor OGR1 (GPR68) Expression and Activation Increases in Intestinal Inflammation and Fibrosis

    doi: 10.3390/ijms23031419

    Figure Lengend Snippet: The OGR1 inhibitor blocked OGR1-associated downstream events in Caco-2 cells and human CD14+ monocytes. ( A ) OGR1 (GPR68) antibody 16H23L16 for immunocytochemical detection of OGR1 was verified in previously validated tools. Caco-2 clone stably overexpressing OGR1 (clone U1); negative control, Caco-2 vector control (VC) cells. Cells were examined by confocal microscopy (Leica, Germany). ( B – F ) Functional assays confirmed pH-dependent OGR1-mediated signalling. ( B ) U1 and VC cells were subjected to an acidic pH shift and intracellular inositol phosphate (IP) formation was measured. Data are presented as mean ± SD and statistical analysis was performed using one-way ANOVA. *** p < 0.001. ( C ) Activated GTPase RhoA was measured in U1 and VC cells upon an acidic pH shift. ( D , E ) Efficacy of the OGR1 inhibitor (GPR68-I) and the enantiomer of the inhibitor were measured using electric cell-substrate impedance-sensing (ECIS) technology. OGR1 clone U1 and VC grown to confluent monolayers were subjected to an acidic pH shift with or without the OGR1 inhibitor (0.5, 1.0, 2.5, 5, 10, 25 µM). Changes in resistance of cell monolayers were monitored in real time. Representative graph of eight independent experiments shown for the inhibitor or the enantiomer. ( F , G ) Human CD14+ monocytes from healthy volunteers and active IBD patients were subjected to an acidic pH shift, with or without the OGR1 inhibitor (0.5, 1.0, 2.5, 5 µM). IP1 production was measured from healthy volunteers ( n = 5) and active IBD patients ( n = 2); active IBD patient ( n = 1). Samples in ( F , G ) were read on the Tecan Infinite or Biotek Synergy instrument, respectively. Data are presented as mean ± SD and statistical analysis was performed using one-way ANOVA. * p < 0.05. IBD: inflammatory bowel disease; OGR1: ovarian cancer G-protein-coupled receptor 1; vector control: VC.

    Article Snippet: Resistance and impedance of Caco-2 monolayers were monitored using electric cell-substrate impedance-sensing (ECIS) instruments (Applied BioPhysics, Troy, NY, USA) as previously described [ ].

    Techniques: Stable Transfection, Negative Control, Plasmid Preparation, Confocal Microscopy, Functional Assay, Electric Cell-substrate Impedance Sensing